SrasV1, Main, Exploration, bibRecord, 001C11

A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities

Identifieur interne : 001C11 ( Main/Exploration ); précédent : 001C10; suivant : 001C12

A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities

Auteurs : PENG LIN [République populaire de Chine] ; JACK HO WONG [République populaire de Chine] ; TZI BUN NG [République populaire de Chine] ; Vincent Sai Man Ho [République populaire de Chine] ; LIXIN XIA [République populaire de Chine]

Source :

Food chemistry [ 0308-8146 ] ; 2013.

RBID : Pascal:14-0111134

Descripteurs français

Pascal (Inist)
- Sorghum, Inhibiteur protéique, Antifongique, Anticancéreux, Virus HIV1, RNA-directed DNA polymerase, Transcription inverse, Protéine, Purification, EC 3.2.1.32, EC 2.7.7.49.

English descriptors

KwdEn :
- Antifungal agent, Antineoplastic agent, EC 2.7.7.49, EC 3.2.1.32, HIV-1 virus, Protein, Protein inhibitor, Purification, RNA-directed DNA polymerase, Reverse transcription, Sorghum.

Abstract

A 25-kDa protein, with an N-terminal amino acid sequence homologous to that of xylanase inhibitor and designated as xylanase inbibitor-like protein (XILP) was purified from sorghum seeds. The isolation protocol consisted of affinity chromatography, ion exchange chromatography, and gel filtration. XILP inhibited mycelial growth in various phytopathogenic fungi. The antifungal activity was thermostable and pH-stable. XILP inhibited proliferation of various cancer cell lines but did not do so in human embryonic liver (WRL 68) cells. There was no mitogenic activity toward mouse splenocytes. XILP reduced the activity of HIV-1 reverse transcriptase with an IC₅₀ of 11.1 μM, but lacked inhibitory activity toward HIV-1 integrase and SARS coronavirus proteinase. In conclusion, sorghum XILP is thermostable and pH stable and exhibits potent antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities.

Affiliations:

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Le document en format XML

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<author><name sortKey="Jack Ho Wong" sort="Jack Ho Wong" uniqKey="Jack Ho Wong" last="Jack Ho Wong">JACK HO WONG</name>
<affiliation wicri:level="4"><inist:fA14 i1="01"><s1>School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin</s1>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal agent</term>
<term>Antineoplastic agent</term>
<term>EC 2.7.7.49</term>
<term>EC 3.2.1.32</term>
<term>HIV-1 virus</term>
<term>Protein</term>
<term>Protein inhibitor</term>
<term>Purification</term>
<term>RNA-directed DNA polymerase</term>
<term>Reverse transcription</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Sorghum</term>
<term>Inhibiteur protéique</term>
<term>Antifongique</term>
<term>Anticancéreux</term>
<term>Virus HIV1</term>
<term>RNA-directed DNA polymerase</term>
<term>Transcription inverse</term>
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<front><div type="abstract" xml:lang="en">A 25-kDa protein, with an N-terminal amino acid sequence homologous to that of xylanase inhibitor and designated as xylanase inbibitor-like protein (XILP) was purified from sorghum seeds. The isolation protocol consisted of affinity chromatography, ion exchange chromatography, and gel filtration. XILP inhibited mycelial growth in various phytopathogenic fungi. The antifungal activity was thermostable and pH-stable. XILP inhibited proliferation of various cancer cell lines but did not do so in human embryonic liver (WRL 68) cells. There was no mitogenic activity toward mouse splenocytes. XILP reduced the activity of HIV-1 reverse transcriptase with an IC<sub>50</sub>
 of 11.1 μM, but lacked inhibitory activity toward HIV-1 integrase and SARS coronavirus proteinase. In conclusion, sorghum XILP is thermostable and pH stable and exhibits potent antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities.</div>
</front>
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<orgName><li>Université chinoise de Hong Kong</li>
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<tree><country name="République populaire de Chine"><noRegion><name sortKey="Peng Lin" sort="Peng Lin" uniqKey="Peng Lin" last="Peng Lin">PENG LIN</name>
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<name sortKey="Jack Ho Wong" sort="Jack Ho Wong" uniqKey="Jack Ho Wong" last="Jack Ho Wong">JACK HO WONG</name>
<name sortKey="Lixin Xia" sort="Lixin Xia" uniqKey="Lixin Xia" last="Lixin Xia">LIXIN XIA</name>
<name sortKey="Sai Man Ho, Vincent" sort="Sai Man Ho, Vincent" uniqKey="Sai Man Ho V" first="Vincent" last="Sai Man Ho">Vincent Sai Man Ho</name>
<name sortKey="Tzi Bun Ng" sort="Tzi Bun Ng" uniqKey="Tzi Bun Ng" last="Tzi Bun Ng">TZI BUN NG</name>
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A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities

A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri